Abstract To explore the effect of evidence-based nursing on the application of ultrasonic emulsification operation for cataract and the postoperative rehabilitation indicators. 110 cataract patients were included. All of them received ultrasonic emulsification operation for cataract and were divided into the control group(n=55) and the observation group(n=55) by random number method. The control group received routine nursing. The visual acuity, intraocular pressure, quality of life and adverse reactions of patients in both groups after discharge were compared and evaluated. The clinical effective rate(92.73%), postoperative visual acuity(0.68 ± 0.05) and SF-36 score(82.37 ± 4.13) in the observation group were higher than those in the control group(76.36%), (0.55 ± 0.02) and(74.06 ± 3.42), Conclusion: Evidence-based nursing can effectively improve the curative effect and postoperative vision, and improve the quality of life. The intraocular pressure in the observation group(9.95 ± 2.46) mmHg and the incidence of adverse reactions(29.09%) were lower than those in the control group(11.03 ± 3.11) mmHg and 10.91%(P<0.05). It is of distinct effect to carry out evidence-based nursing for the cataract patients receiving emulsification therapy. It effectively improves the effect of surgical treatment, reduce the occurrence of adverse reactions, so as to improve the quality of life and promote rapid physical recovery, maintaining clinical application value.

Resumo Introdução: A lesão hepática por isquemia-reperfusão é um processo fisiopatológico comum em cirurgias hepáticas. Mais estudos ainda são necessários para avaliar se o propofol pode reduzir a lesão de isquemia-reperfusão miocárdica induzida pela lesão de isquemia-reperfusão hepática em ratos, juntamente com os mecanismos que estão relacionados. Objetivo: Investigar se propofol protege as células do miocárdio da apoptose com a lesão hepática por isquemia-reperfusão. Métodos: Ratos machos da raça Sprague-Dawley (n = 18) foram alocados aleatoriamente em três grupos: Grupo Sham (Grupo S, n = 6), Grupo Lesão Hepática por Isquemia-reperfusão (Grupo IR, n = 6) e Grupo Propofol (Grupo P, n = 6). O Grupo S foi submetido apenas à laparotomia. O grupo IR foi submetido à isquemia por 30 min e reperfusão por 4 h. O grupo P foi submetido à mesma isquemia do grupo IR, com a administração de 120 mg.kg-1 de propofol iniciada 10min antes da isquemia, seguida de infusão contínua a 20 mg.kg-1.h-1. A apoptose celular foi examinada por meio do ensaio de marcação de terminações dUTP pela deoxinucleotidil transferase. Retículo endoplasmático Ca2+-ATPase2 (SERCA2) e caspase-3 do ácido aspártico contendo cisteína (caspase-3 clivada) foram avaliados com o ensaio western blot e reação em cadeia da polimerase. Resultados: A taxa de apoptose foi maior com mRNA e proteína de SERCA2 regulados para baixo e caspase-3 clivada suprarregulada no Grupo IR, em comparação com o Grupo S (p < 0,01). A taxa de apoptose foi menor com mRNA e proteína de SERCA2 suprarregulada e caspase-3 clivada sub-regulada no Grupo P, em comparação com o Grupo IR (p < 0,01). Conclusões: O propofol pode reduzir a apoptose de células miocárdicas induzida por lesão hepática por isquemia-reperfusão. Entretanto, pode suprarregular o mRNA e a proteína de SERCA2 em ratos.

Abstract Introduction: Hepatic ischemia-reperfusion injury is a common pathophysiological process in liver surgery. Whether Propofol can reduce myocardial ischemia-reperfusion injury induced by hepatic ischemia-reperfusion injury in rats, together with related mechanisms, still needs further studies. Objective: To investigate if propofol would protect the myocardial cells from apoptosis with hepatic ischemia-reperfusion injury. Methods: Male Sprague-Dawley rats (n = 18) were randomly allocated into three groups: Sham Group (Group S, n = 6), Hepatic Ischemia-reperfusion Injury Group (Group IR, n = 6) and Propofol Group (Group P, n = 6). Group S was only subjected to laparotomy. Group IR was attained by ischemia for 30 min and reperfusion for 4 h. Group P was subjected identical insult as in Group IR with the administration of propofol started 10 min before ischemia with 120 mg.kg−1, following by continuous infusion at 20 mg.kg−1.h−1. Cell apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay. Endoplasmic reticulum Ca2+-ATPase2 (SERCA2) and cysteine-containing aspartic acid cleaved-caspase3 (cleaved-caspase3) were assayed by western blot and Altimeter polymerase chain reaction. Results: Apoptosis rate was increased, with mRNA and protein of SERCA2 down-regulated and cleaved-caspase3 up-regulated in Group IR compared with Group S (p < 0.01). Apoptosis rate was decreased, with mRNA and protein of SERCA2 up-regulated and cleaved-caspase3 down-regulated in Group P compared with Group IR (p < 0.01). Conclusions: Propofol can reduce hepatic ischemia-reperfusion injury-induced myocardial cell apoptosis, meanwhile, can up-regulate mRNA and protein of SERCA2 in rats.

OBJECTIVE: To investigate the association between pesticide exposure and suicidal ideation in rural areas of China. METHODS: The analysis involved data from a survey of a representative sample of 9811 rural residents in Zhejiang province who had been asked about the storage of pesticides at home and about whether or not they had considered suicide within the 2 years before the interview. The Chinese version of the 12-item General Health Questionnaire (GHQ) was administered to screen for mental disorder. FINDINGS: The unadjusted odds ratio (OR) for the association between pesticide storage at home and suicidal ideation over the prior 2 years was 2.12 (95% confidence interval, CI: 1.54-2.93). After adjusting for gender, age, education, socioeconomic status, marital status, physical health, family history of suicidal behaviour, GHQ caseness and study design effects, the OR was 1.63 (95% CI: 1.13-2.35). CONCLUSION: A potential marker of chronic pesticide exposure was found to be associated with suicidal ideation, which supports findings from previous studies. Given the high level of pesticide exposure and the high suicide risk in rural China, clarification of the causal mechanisms underlying this association and the development of appropriate interventions are priorities for public health and health policy.

OBJETIVO: Investigar la relación entre la exposición a plaguicidas y las ideas suicidas en zonas rurales de China. MÉTODOS: Este análisis se basó en los datos de una encuesta de una muestra representativa de 9811 habitantes de zonas rurales de la provincia de Zhejiang a quienes se les preguntó si almacenaban plaguicidas en su hogar y si en los dos últimos años habían considerado en algún momento la posibilidad de suicidarse. A fin de detectar posibles trastornos mentales, se utilizó también la versión en chino del Cuestionario de Salud General (GHQ) de 12 ítems. RESULTADOS: La oportunidad relativa (OR) no ajustada para la relación entre el almacenamiento de plaguicidas en el domicilio y las ideas suicidas durante los dos años precedentes fue del 2,12% (intervalo de confianza [IC] del 95%: 1,54-2,93). Después de ajustar en función del sexo, la edad, la educación, el nivel socioeconómico, el estado civil, la salud física, los antecedentes familiares de comportamiento suicida, las puntuaciones obtenidas en el GHQ y los efectos del diseño del estudio, la OR fue de 1,63 (IC95%: 1,13-2,35). CONCLUSIÓN: Se ha detectado una relación significativa entre un posible marcador de la exposición crónica a plaguicidas y las ideas suicidas, lo que avala los resultados de estudios anteriores. Considerando el alto nivel de exposición a plaguicidas y el alto riesgo de suicidio que se dan en la China rural, el esclarecimiento de los mecanismos causales de esa relación y el desarrollo de las intervenciones oportunas son una prioridad para la salud pública y las políticas sanitarias.

OBJECTIF: Étudier l'association entre l'exposition aux pesticides et les idées suicidaires dans les zones rurales chinoises. MÉTHODES: L'analyse a porté sur les données d'une enquête sur un échantillon représentatif de 9811 habitants ruraux de la province du Zhejiang auxquels on a demandé s'ils stockaient des pesticides à domicile et s'ils avaient envisagé de se suicider dans les deux ans précédant l'entretien. La version chinoise du Questionnaire général de santé en 12 points pour les troubles mentaux (GHQ) a été administrée pour dépister les éventuels troubles mentaux. RÉSULTATS: L'Odds ratio (OR) non ajusté pour l'association entre le stockage de pesticides à domicile et les idées suicidaires sur la période antérieure de 2 ans était de 2,12 (intervalle de confiance à 95 %, IC : 1,54-2,93). Après ajustement pour le sexe, l'âge, le niveau d'éducation, le statut socioéconomique, le statut marital, la santé physique, les antécédents familiaux de comportement suicidaire, la susceptibilité d'être atteint de troubles mentaux et les effets du type d'étude, l'OR était de 1,63 (IC à 95 % : 1,13-2,35). CONCLUSION: On a constaté qu'un marqueur possible de l'exposition chronique aux pesticides était associé aux idées suicidaires, ce qui confirme les résultats des études antérieures. Compte tenu de la forte intensité de l'exposition aux pesticides et du risque élevé de suicide en Chine rurale, l'explicitation des mécanismes causals sous-jacents à cette association et le développement d'interventions appropriées sont des priorités en matière de santé publique et de politique sanitaire.